Expression and activity of a multixenobiotic resistance system in the Pacific oyster Crassostrea gigas.
Identifieur interne : 003328 ( Main/Exploration ); précédent : 003327; suivant : 003329Expression and activity of a multixenobiotic resistance system in the Pacific oyster Crassostrea gigas.
Auteurs : C. Minier [France] ; C. Lelong ; N. Djemel ; F. Rodet ; R. Tutundjian ; P. Favrel ; M. Mathieu ; F. LeboulengerSource :
- Marine environmental research [ 0141-1136 ]
English descriptors
- KwdEn :
- Animals, Coloring Agents (pharmacokinetics), Drug Resistance, Multiple, Gene Expression Regulation, Genes, MDR (genetics), Gills (physiology), Larva, Ostreidae (genetics), Ostreidae (growth & development), Ostreidae (physiology), Reverse Transcriptase Polymerase Chain Reaction (veterinary), Rhodamines (pharmacokinetics), Tissue Distribution, Water Pollutants, Chemical (adverse effects), Water Pollutants, Chemical (metabolism), Xenobiotics (adverse effects), Xenobiotics (metabolism).
- MESH :
- chemical , adverse effects : Water Pollutants, Chemical, Xenobiotics.
- chemical , metabolism : Water Pollutants, Chemical, Xenobiotics.
- chemical , pharmacokinetics : Coloring Agents, Rhodamines.
- genetics : Genes, MDR, Ostreidae.
- growth & development : Ostreidae.
- physiology : Gills, Ostreidae.
- veterinary : Reverse Transcriptase Polymerase Chain Reaction.
- Animals, Drug Resistance, Multiple, Gene Expression Regulation, Larva, Tissue Distribution.
Abstract
The multidrug resistance (MDR) mechanism corresponds to a defence system relying on the expression of high molecular membrane proteins that can actively lower the intracellular concentration of a wide variety of toxins, thus maintaining them below their toxic level. Using RT-PCR, expression levels of a gene belonging to the class I of mammalian mdr genes, has been assessed in different developmental stages of the oyster Crassostrea gigas. While no expression was found in the oocyte or the trocophore stage, a rise of mRNA content was observed from the veliger stage to the juvenile stage, thus indicating the induction of the system as the animal is developing in the environment. The incubation of gill fragments in the dye rhodamine B and subsequent measurements of intracellular fluorescence using a microplate reader indicates that the system can effectively decrease the accumulation of the test compound in a competitive manner with known inhibitors or environmental contaminants as observed in vertebrate cells. The oyster MXR system is thus becoming active in adult oyster and could be of importance in environmentally contaminated areas.
PubMed: 12408601
Affiliations:
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Le document en format XML
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Djemel</name></author><author><name sortKey="Rodet, F" sort="Rodet, F" uniqKey="Rodet F" first="F" last="Rodet">F. Rodet</name></author><author><name sortKey="Tutundjian, R" sort="Tutundjian, R" uniqKey="Tutundjian R" first="R" last="Tutundjian">R. Tutundjian</name></author><author><name sortKey="Favrel, P" sort="Favrel, P" uniqKey="Favrel P" first="P" last="Favrel">P. Favrel</name></author><author><name sortKey="Mathieu, M" sort="Mathieu, M" uniqKey="Mathieu M" first="M" last="Mathieu">M. Mathieu</name></author><author><name sortKey="Leboulenger, F" sort="Leboulenger, F" uniqKey="Leboulenger F" first="F" last="Leboulenger">F. Leboulenger</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="????"><PubDate><MedlineDate>2002 Sep-Dec</MedlineDate></PubDate></date><idno type="RBID">pubmed:12408601</idno><idno type="pmid">12408601</idno><idno type="wicri:Area/PubMed/Corpus">000367</idno><idno type="wicri:Area/PubMed/Curation">000367</idno><idno type="wicri:Area/PubMed/Checkpoint">000367</idno><idno type="wicri:Area/Ncbi/Merge">000058</idno><idno type="wicri:Area/Ncbi/Curation">000058</idno><idno type="wicri:Area/Ncbi/Checkpoint">000058</idno><idno type="wicri:doubleKey">0141-1136::Minier C:expression:and:activity</idno><idno type="wicri:Area/Main/Merge">003415</idno><idno type="wicri:Area/Main/Curation">003328</idno><idno type="wicri:Area/Main/Exploration">003328</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Expression and activity of a multixenobiotic resistance system in the Pacific oyster Crassostrea gigas.</title><author><name sortKey="Minier, C" sort="Minier, C" uniqKey="Minier C" first="C" last="Minier">C. Minier</name><affiliation wicri:level="1"><nlm:affiliation>LEMA, UPRES-EA 3222, University of Le Havre, France. minier@univ-lehavre.fr</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>LEMA, UPRES-EA 3222, University of Le Havre</wicri:regionArea><wicri:noRegion>University of Le Havre</wicri:noRegion><wicri:noRegion>University of Le Havre</wicri:noRegion></affiliation></author><author><name sortKey="Lelong, C" sort="Lelong, C" uniqKey="Lelong C" first="C" last="Lelong">C. Lelong</name></author><author><name sortKey="Djemel, N" sort="Djemel, N" uniqKey="Djemel N" first="N" last="Djemel">N. Djemel</name></author><author><name sortKey="Rodet, F" sort="Rodet, F" uniqKey="Rodet F" first="F" last="Rodet">F. Rodet</name></author><author><name sortKey="Tutundjian, R" sort="Tutundjian, R" uniqKey="Tutundjian R" first="R" last="Tutundjian">R. Tutundjian</name></author><author><name sortKey="Favrel, P" sort="Favrel, P" uniqKey="Favrel P" first="P" last="Favrel">P. Favrel</name></author><author><name sortKey="Mathieu, M" sort="Mathieu, M" uniqKey="Mathieu M" first="M" last="Mathieu">M. Mathieu</name></author><author><name sortKey="Leboulenger, F" sort="Leboulenger, F" uniqKey="Leboulenger F" first="F" last="Leboulenger">F. Leboulenger</name></author></analytic><series><title level="j">Marine environmental research</title><idno type="ISSN">0141-1136</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Coloring Agents (pharmacokinetics)</term><term>Drug Resistance, Multiple</term><term>Gene Expression Regulation</term><term>Genes, MDR (genetics)</term><term>Gills (physiology)</term><term>Larva</term><term>Ostreidae (genetics)</term><term>Ostreidae (growth & development)</term><term>Ostreidae (physiology)</term><term>Reverse Transcriptase Polymerase Chain Reaction (veterinary)</term><term>Rhodamines (pharmacokinetics)</term><term>Tissue Distribution</term><term>Water Pollutants, Chemical (adverse effects)</term><term>Water Pollutants, Chemical (metabolism)</term><term>Xenobiotics (adverse effects)</term><term>Xenobiotics (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="adverse effects" xml:lang="en"><term>Water Pollutants, Chemical</term><term>Xenobiotics</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Water Pollutants, Chemical</term><term>Xenobiotics</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacokinetics" xml:lang="en"><term>Coloring Agents</term><term>Rhodamines</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Genes, MDR</term><term>Ostreidae</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Ostreidae</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Gills</term><term>Ostreidae</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Drug Resistance, Multiple</term><term>Gene Expression Regulation</term><term>Larva</term><term>Tissue Distribution</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The multidrug resistance (MDR) mechanism corresponds to a defence system relying on the expression of high molecular membrane proteins that can actively lower the intracellular concentration of a wide variety of toxins, thus maintaining them below their toxic level. Using RT-PCR, expression levels of a gene belonging to the class I of mammalian mdr genes, has been assessed in different developmental stages of the oyster Crassostrea gigas. While no expression was found in the oocyte or the trocophore stage, a rise of mRNA content was observed from the veliger stage to the juvenile stage, thus indicating the induction of the system as the animal is developing in the environment. The incubation of gill fragments in the dye rhodamine B and subsequent measurements of intracellular fluorescence using a microplate reader indicates that the system can effectively decrease the accumulation of the test compound in a competitive manner with known inhibitors or environmental contaminants as observed in vertebrate cells. The oyster MXR system is thus becoming active in adult oyster and could be of importance in environmentally contaminated areas.</div></front></TEI><affiliations><list><country><li>France</li></country></list><tree><noCountry><name sortKey="Djemel, N" sort="Djemel, N" uniqKey="Djemel N" first="N" last="Djemel">N. Djemel</name><name sortKey="Favrel, P" sort="Favrel, P" uniqKey="Favrel P" first="P" last="Favrel">P. Favrel</name><name sortKey="Leboulenger, F" sort="Leboulenger, F" uniqKey="Leboulenger F" first="F" last="Leboulenger">F. Leboulenger</name><name sortKey="Lelong, C" sort="Lelong, C" uniqKey="Lelong C" first="C" last="Lelong">C. Lelong</name><name sortKey="Mathieu, M" sort="Mathieu, M" uniqKey="Mathieu M" first="M" last="Mathieu">M. Mathieu</name><name sortKey="Rodet, F" sort="Rodet, F" uniqKey="Rodet F" first="F" last="Rodet">F. Rodet</name><name sortKey="Tutundjian, R" sort="Tutundjian, R" uniqKey="Tutundjian R" first="R" last="Tutundjian">R. Tutundjian</name></noCountry><country name="France"><noRegion><name sortKey="Minier, C" sort="Minier, C" uniqKey="Minier C" first="C" last="Minier">C. Minier</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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